Poolseq data from two hybrid wood ant lineages

This data is linked to the following preprint: https://doi.org/10.1101/500116. Whole-genome pooled sequencing was used to compare male and female genomes between two hybrid wood ant lineages (W and R) and discover candidate male barrier loci. Samples were collected from the L?ngholmen hybrid population (10.1073/pnas.0912409107) in the year 2004. The samples were freshly frozen and kept in -20?C until genomic DNA was extracted in the year 2010 from half a body using a Qiagen kit. Four pools were sequenced, each consisting of 24 individuals: R males, R females (unmated queens), W males and W females (unmated queens). These individuals were classified into the two lineages based on six to seven diagnostic microsatellite alleles (10.1073/pnas.0912409107). Individual DNA concentrations were checked with Qubit and equal amounts were combined to create the four pools. Due to haplodiploidy, this resulted in 24 and 48 chromosomes sampled for male and female pools, respectively. These pools represent population samples and came from two to four nests each, all of which belong to the same supercolony, where nests have hundreds of reproductive queens and relatedness approaching zero.