An Improved Protocol for the Synthesis and Purification of Yariv Reagents

Yariv
reagents are glycoconjugate tris-azo dyes widely used in
plant biology. These reagents are synthesized by diazo coupling between
phloroglucinol and a para-diazophenyl glycoside.
Despite their synthetic accessibility, well-defined protocols for
obtaining pure Yariv reagents, and their complete compound characterization
data, have not been reported. We report here optimized protocols used
to synthesize, purify, and characterize a panel of six Yariv reagents
and suggest approaches that could be valuable for the purification
and characterization of other glycoconjugates as well.