Effect of extracellular matrix extract of decellularized extracellular matrix material on immune characteristics of RAW264.7 macrophages

Objective This study aims to investigate how extracellular matrix material extract affects macrophage activity, migration, phagocytosis, the expression of proinflammatory factors, and ROS production.Methods We assessed the effect of dECM extract on macrophage activity using the CCK-8 method under a light microscope. We used the Transwell migration assay to evaluate the effects of dECM extracts on the recruitment and chemotaxis of macrophages. pHrodo staining was employed to examine the impact of dECM extracts on macrophage phagocytosis. We utilized RT-qPCR and immunofluorescence staining to assess how dECM extract affects the expression of proinflammatory genes in macrophages. Additionally, we examined the expression of specific proinflammatory functional molecules. The DCFH-DA fluorescent probe method was used to detect the effect of dECM material extract on ROS production in macrophages.Results Compared with DMEM complete medium, CCK-8 results showed that dECM material extract could increase the activity of macrophages; RT-qPCR results indicated that dECM extracts could down-regulate the gene expression of proinflammatory cytokines and specific functional molecules; Flow cytometry results demonstrated that the extract of dECM material could reduce the production of ROS by macrophages. The results indicated that the dECM material extract exhibited favorable compatibility and beneficial biological properties for macrophages.