Transcriptional Profiling of Ngn3-dependent endocrine progenitors and pre-beta cells

The goal of this study was to examine genetic networks that control endocrine cell type specification and differentiation. RNA-Seq technology was used to explore the gene expression profiles of pancreatic endocrine progenitor cells (at E10.5 and E15.5), impaired endocrine progenitor cells (at E10.5 and E15.5), and pre-beta cells (at E15.5) in mouse.本研究旨在解析调控内分泌细胞类型特化与分化的基因调控网络。本研究采用RNA测序（RNA-Seq）技术，对小鼠体内三类细胞的基因表达谱进行探究：分别为胚胎发育第10.5天（E10.5）与第15.5天（E15.5）的胰腺内分泌祖细胞、同期的功能受损胰腺内分泌祖细胞，以及胚胎发育第15.5天（E15.5）的前β细胞。