Remodeling of cell-type specific transcriptomes upon T cell stimulation in Systemic lupus erythematosus versus Sjögren's syndrome

The RNA-sequencing to profile patient peripheral blood mononuclear cell (PBMC) samples of systemic lupus erythematosus (SLE, N=25), primary Sjögren's syndrome (pSS, N=23), and healthy control (HD, N=24). We collected on the steady-state and 18-hours anti-CD3 culture to identify the transcriptome remodeling upon T-cell stimulation. Each patient samples were sorted for CD4+ (Th cells) and CD8+ (CTL) T lymphocytes, CD16+CD7+ cells (NK cells), and CD19+ cells (B cells).