Data from: Evolution of flexible biting in hyperdiverse parasitoid wasps
收藏Mendeley Data2024-04-13 更新2024-06-29 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.0rxwdbs1x
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Tomographic scans of ethanol-preserved insect heads were performed at the UFO-I station of the Imaging Cluster at the KIT light source using a parallel polychromatic X-ray beam produced by a 1.5 T bending magnet. The beam was spectrally filtered by 0.5 mm aluminum and the resulting spectrum had a peak at about 15 keV, with a full-width at half maximum bandwidth of about 10 keV. A fast indirect detector system was employed, consisting of a 12 µm LSO:Tb scintillator and a diffraction limited optical microscope (Optique Peter) coupled with a 12bit pco.dimax high speed camera with 2016 x 2016 pixels. Scans were done by taking 3,000 projections at 70 fps and an optical magnification of 10x, resulting in an effective pixel size of 1.22 µm. We used the control system concert for automated data acquisition and online reconstruction of tomographic slices for data quality assurance. Online and final data processing included flat field correction and phase retrieval of the projections based on the transport of intensity equation. X-ray beam parameters for algorithms in the data processing pipeline were computed by syris and the execution of the pipelines, including tomographic reconstruction, was performed by the UFO framework.
创建时间:
2023-06-28



