Lethal phases of flw alleles and rescue data.

Note: Lethal phase is defined as the maximum stage the animals can reach. Some animals die at earlier stages, and if the culture is too crowded, the lethal phase can be earlier. The lethal phases of flw6, flw7, and flwFP41 are listed as already published [23]-[25]. In addition, since the eggs for lethal phase analysis were from heterozygous females, maternal contributions were included. Maternal contributions cannot be avoided because Flw is required for oogenesis. All alleles except XE55C were rescued to fertile adults with an flw-carrying duplication Dp(1;3)DC224.*: XE55C itself was not rescued, but XE55C females transheterozygous with XE55D or XE55E were rescued by Dp(1;3)DC224 to fertile adults. This suggests that there is a second non-flw lethal hit on the XE55C-carrying chromosome.For rescues with Pp1ß9C (flw cDNA under the control of UASt promoter, which expresses Flw-pB in somatic cells specified by Gal4 lines used [23], [24]), the following Gal4 lines were used: tub-Gal4 (w; UAS-Pp1ß9C/CyO; TubGal4/TM6C) expressing Gal4 under the control of the α-tubulin84B promoter, maternal Gal4 (w*; P{w[+mC] = matalpha4-GAL-VP16}V37) expressing GAL4-VP16 fusion protein under the control of the α-tubulin67C promoter, act Gal4-II (y1w*; P{w[+mC] = Act5C-GAL4}25FO1/CyO, y+) and act Gal4-III (y1w*; P{w[+mC] = Act5C-GAL4}25FO1/CyO, y+) expressing Gal4 under the control of actin5C promoter. For rescuing with tubGal4, males of w; UAS-Pp1ß9C/CyO; TubGal4/TM6C were crossed with females of flwmutations/FM7. For other Gal4-UASt-Pp1ß9C mediated rescues, females of flwmutations/FM7; UASPp1ß9C/CyO were crossed with males of corresponding Gal4 lines. For rescuing with duplication, males carrying Dp(1;3)DC224 were crossed with females of flwmutations/FM7. For female rescues, rescued XE55 mutant males were crossed with females of flwmutations/FM7. NA: not available.