Integrator-PP2A complex regulates transcription via dephosphorylating RNA Polymerase II

The phosphorylation and dephosphorylation of transcription machinery are essential for the precise control of gene expression. A non-canonical protein phosphatase 2A (PP2A) holoenzyme (denoted INTAC), in which the 14-subunit Integrator recruits RNA polymerase II (Pol II) and the PP2A core enzyme dephosphorylates the C-terminal repeat domain (CTD) of Pol II at Serine-5 and Serine-2. Overall design: We have finished the ChIP-seq and nascent-seq to investigate the role of integrated phosphatase and endonuclease activities in this Integrator-PP2A complex and its function in regulating Pol II phosphorylation and transcription.