Steady-state interactions detected using co-IP did not reflect the compatibility between Rpi-blb2 and NRC3 variants.
收藏NIAID Data Ecosystem2026-05-02 收录
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Co-immunoprecipitation assays of Rpi-blb2 and NRC3 variants. (A) RFP-Rpi-blb2 and NRC3s-myc were co-expressed with or without Flag-AVRblb2. NRC3s-myc co-expressed with RFP were used as negative controls. Protein extracts (input) and RFP-Trap pull-down samples (IP) were analyzed using western blot analysis with α-RFP, and α-myc antibodies. SimplyBlue SafeStain-staining of Rubisco was used as the loading control. (B) Protein extracts (input) and myc-Trap pull-down samples (IP) were analyzed using western blot analysis using α-RFP, and α-myc antibodies. SimplyBlue SafeStain-staining of Rubisco was used as the loading control. (JPF)
创建时间：
2024-09-12
