File S1 - Hedgehog-PKA Signaling and gnrh3 Regulate the Development of Zebrafish gnrh3 Neurons

Contains Figures S1, S2, and S3. Figure S1. Similar patterns between LacZ staining and GFP signals at different stages. Figure S2. Structure of the gnrh3 mRNA, its gene, and the design of plasmid to generate transgenic fish expressing GFP-LacZ under the control of the gnrh3 promoter. Figure S3. PKI rescue the gnrh3 neurons in oep or cyc mutants at 30 hpf. A, Examination of gnrh3 cell numbers in PKI-injected oep mutant. B, Examination of gnrh3 cell numbers in PKI-injected cyc mutant. (DOCX)