Dissection of the effects of NUP98-HOXA9 on hematopoietic differentiation based on cell morphology.

*P<0.05. **P<0.01. Averages from 3–4 independent experiments are shown; cells from 2 CFC plates for each experimental condition were harvested. The bottom line shows the average total cell numbers in the two plates±standard deviations. Cytospins smears were prepared and stained with Giemsa; a 500 cell differential count was performed. Cells with blast and promyelocyte morphology were counted as primitive; those with myelocyte/metamyelocyte morphology as intermediate myeloid; those with band, segmented neutrophil, monocyte, and macrophage morphology as mature myeloid; those with intermediate hemoglobinization as intermediate erythroid; and those with full hemoglobinization as mature erythroid. The first 5 rows show average percentages±standard deviations. The P value was obtained by comparing to control using a paired two-tailed distribution t-test.