Table_1_Proteomic analysis of the serum in dogs with pulmonary hypertension secondary to myxomatous mitral valve disease: the preliminary study.XLSX

BackgroundPulmonary hypertension (PH) is a common complication in dogs with myxomatous mitral valve disease (MMVD), characterized by elevated blood pressure in pulmonary artery. Echocardiography is a reliable technique for PH diagnosis in veterinary medicine. However, it is limited to use as an early detection method. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) has found extensive application in the discovery of serum protein biomarkers for various diseases. The objective of this study was to identify serum proteins in healthy control dogs and MMVD dogs both with and without PH using LC–MS/MS.Materials and methodsIn this research, a total of 81 small-breed dogs participated, and they were categorized into three groups: the control (n = 28), MMVD (n = 24) and MMVD+PH (n = 29) groups. Serum samples were collected and analyzed by LC–MS/MS.ResultsDifferentially expressed proteins were identified, and the upregulated and downregulated proteins in MMVD+PH group including Myomesin 1 (MYOM1) and Histone deacetylase 7 (HDAC7), Pleckstrin homology domain containing M3 (PLEKHM3), Diacylglycerol lipase alpha (DAGLA) and Tubulin tyrosine ligase like 6 (TTLL6) were selected as proteins of interest in MMVD dogs with PH.ConclusionDifferent types of proteins have been identified in healthy dogs and MMVD dogs with and without PH. Additional studies are needed to investigate the potential of these proteins as biomarkers for PH in dogs with MMVD.

背景肺动脉高压（PH）是犬类混合性二尖瓣疾病（MMVD）的常见并发症，其特征为肺动脉血压升高。超声心动图是兽医医学中PH诊断的可靠技术，但其应用限于早期检测。液相色谱-串联质谱法（LC-MS/MS）在多种疾病血清蛋白生物标志物的发现中得到了广泛的应用。本研究的目的是利用LC-MS/MS技术，鉴定健康对照组犬和MMVD犬（包括伴有和未伴有PH的犬）血清蛋白。研究材料与方法在本研究中，共有81只小型犬参与，它们被分为三组：对照组（n=28）、MMVD组（n=24）和MMVD+PH组（n=29）。血清样本被收集并通过LC-MS/MS进行分析。研究结果在差异表达蛋白中确定了具有兴趣的蛋白，包括在MMVD+PH组中上调的肌球蛋白1（MYOM1）和组蛋白脱乙酰化酶7（HDAC7）、磷脂酰肌醇同源结构域含有M3（PLEKHM3）、二酰甘油脂酶α（DAGLA）和微管酪氨酸连接酶样6（TTLL6）。结论在健康犬、伴有PH的MMVD犬以及未伴有PH的MMVD犬中，均识别出不同类型的蛋白。为进一步探究这些蛋白作为MMVD犬PH生物标志物的潜力，需要进一步的研究。