Lambertia multiflora microsatellite data

The restoration of diverse self-sustaining ecosystems requires re-establishment of functional interactions among species. For plant communities, pollinators are usually essential for pollination, seed set, and seed quality. A common assumption in ecological restoration for plants pollinated by animals is one of “build it and they will come”, which is rarely tested. Beyond seed set, there may be negative genetic consequences for seed quality if pollinators and their behaviour do not reflect those in reference populations. Here, we conduct an ecological genetic assessment of seed quality via mating system parameters in Lambertia multiflora(Proteaceae), a species dependent on nectar-feeding birds for pollination. Four populations of L. multiflora in disturbed sites that were rehabilitated following mineral sand mining were compared to four natural reference populations, near Eneabba, Western Australia. In each population, approximately 10 offspring from each of 10 maternal plants were geno..., Study populations and sampling
We identified eight populations of L. multiflora where plants were common and of equivalent size, and populations were of equivalent size and density (range 0.1-0.6 plants/m2). Four populations were in post-mining restoration sites, two older (established 30 and 38 years old) and two younger (established 10 and 11 years old), as well as four nearby undisturbed natural (reference) populations (Suppl Table 1; Fig 2). At all restoration sites, seed of L. multiflora have originated from topsoil or mulch, which has originated from multiple sources across the mine lease. From each population, we arbitrarily selected 10 maternal plants that were spaced >5 m apart and were fruit bearing, from which we collected at least 20 seed-bearing fruit per maternal. Leaves were sampled from all maternal plants and stored in activated silica for extraction of DNA. Seed were extracted from fruits, weighed and germinated, and leaves sampled from seedlings grown in the glassh..., Excel, Data file is an Excel spreadsheet containing microsatellite data for 682 samples of Lambertia multiflora offspring from 8 populations, 4 natural reference populations (N1-4) and 4 restored populations (R1-4).

Data are formatted for analysis in the software Genalex (Peakall, R. & Smouse, P. E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. *Molecular Ecology Notes, 6*, 288-295; Peakall, R.& Smouse, P.E. (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research – an update. *Bioinformatics 28*, 2537-2539); [https://biology-assets.anu.edu.au/GenAlEx/Welcome.html](https://biology-assets.anu.edu.au/GenAlEx/Welcome.html)

Data are as per Genalex formatting requirements:

Row 1: # of loci (11), # of individuals (682), # of populations (8), then number of individuals from each of 8 populations.

Row 2: title, then names of each of 8 populations (R1-4, N1-4), where R refers to a restored po...