Comparison of Theory and Experiments: Oscillation Pattern after Microtubule Cutting

(A�CC) Numerical results and (D�CF) experimental measurements of the SPB position as a function of time. The red bolt signs mark the time and the SPB position at the moment of microtubule cutting. Schemes show the SPB and the microtubules, as well as the site of laser ablation (bolt signs); the black arrows indicate the direction of SPB movement. Laser ablation of the leading microtubules induced a change of direction of the SPB movement (red arrows in [A and D]). In experiments, the change occurred within 25 �� 13 s after the ablation (mean �� standard deviation, n = 12). Laser ablation of the trailing microtubules (B, E) or the tip of the leading microtubules (C, F) did not perturb the SPB movement. The parameters in (A�CC) are as in Figure 4, except ��0 = 5 ��m/min, ��s = ?5 ��m/min, and ��g = 3 ��m/min corresponding to the strain SV28xSV31 (Text S1, Table SIII). The gray rectangles in (D�CF) mark the time interval of the images shown in Figures 1B�C1D; the green lines mark the cell ends.