A Novel Cereblon Modulator Targets eRF3 Family Proteins For Cereblon-dependent Destruction

To discern why GSPT1 depletion induced growth inhibition, we utilized RNA microarray analysis to profile the transcriptional changes in cells treated with CC-885 or DMSO. To discern why CC-885 induced growth inhibition, we utilized RNA microarray analysis to profile the transcriptional changes in cells treated with CC-885 or DMSO Several key markers of the ATF4 pathway including ATF3, CHOP (DDIT3), and ATF4 were significantly induced by CC-885 treatment. Human breast cancer cell line (T47D) were treated with CC-15885 or DMSO for 1,2,4, or 8hr in replicate. The RNA samples were assayed on Affymetrix microarrays.