Solanum lycopersicum Transcriptome or Gene expression
收藏NIAID Data Ecosystem2026-03-11 收录
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资源简介：
Solanum lycopersicum RNA degradome sequencing Overall design: Isolated polyadenylated RNA from total RNA extracts of Solanum lycopersicum, were ligated to 5'-adapter that includes an MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and ligated to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.
创建时间：
2019-09-23
