Differential gene expression Normal lymph nodes vs Metastatic lymph nodes

Differential expressionwas assessed for each gene using an exact test analogous to Fisher's exact test butadapted for overdispersed data. TMM normalization and Tagwise dispersion (individual dispersion for each gene) were used to adjust for abundance differences across samples, and DEGs were extracted. Genes were considered differentially expressed only at a P-value <0.05 and at False Discovery Rate (FDR) < 0.05. Genes with log2-fold changes of more than 2 or less than -2 were then selected to ensure that only robust changes were considered.Differentially expressed genes between two groups were annotated using HUGO Gene Nomenclature Committee^TM(HGNC) (https://www.genenames.org).