Gut alterations in a chronic kidney disease rat model with diet-induced vascular calcification

Intestinal disorders and vascular calcification (VC) are often associated with chronic kidney disease (CKD). While gut barrier alterations have been reported in CKD (abnormal intestinal permeability, bacterial overgrowth and inflammation), it is not clear if vascular calcification influences such alterations. Bidirectional relationships between VC and gut dysfunction could be mediated by increased inflammation and uremic toxin generation. We used the SNx-VC model of uremic vascular calcification (rats undergoing subtotal 5/6th nephrectomy and fed a pro-calcifying high-phosphate and vitamin D diet) to investigate gut alterations in vivo. We confirmed the presence of CKD and VC (von Kossa), and observed increased gut-origin uremic toxin, indoxyl sulfate (IS), in SNx-VC animals compared to controls. In SNx-VC, we observed a decreased mucus production (Alcian blue, Mucin 2 staining) in colon and ileum that correlated with calcification severity. There was no change in inflammation markers o..., , , # Gut alterations in a chronic kidney disease rat model with diet-induced vascular calcification

### DOI Links

This dataset contains all the data analysed in the related manuscript. For methods and statistical analyses, please refer to the manuscript.

* The dataset: [https://doi.org/10.5061/dryad.k0p2ngfhx](https://doi.org/10.5061/dryad.k0p2ngfhx)
* The article: [https://doi.org/10.1002/2211-5463.70043](https://doi.org/10.1002/2211-5463.70043)

## Description of the data 

### File

* Gut_alterations_in_SNXVC_rat.csv

### Structure

* Each line represents one observational unit (one animal).
* The columns refer to the experimental groups and individual values observed at the end of protocol.
* Individual values correspond to blood, urine, kidney, aorta, colon, and ileum analysis (biological assays, histology, RTqPCR).
* Values are reported in original scale and, if applicable, after Transformation.
* Missing values are marked as \".\"

### Variables

| Name ...,