Doubling time and plating efficiency of the mutants.
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A colony from each mutant strains and one for each wild-type strain were inoculated in MM medium at 25°C and 32°C at 106 cells/mL (Coulter 5000). Growth was followed at different times by measuring cell concentration with a Beckman Coulter Z1. Linear regression in the exponential growth was used to determine the doubling time. Growing cultures were plated out on three MM plates at 150 colonies per plate at both 25°C and 32°C. Colonies formed after incubation were counted and plating efficiency calculated. (XLSX)
创建时间:
2026-03-06



