Genome-wide chromatin and gene expression profiling during memory formation and maintenance in adult mice. [Data Record 1: qPCR validations]

<b>DESCRIPTION</b><br>qPCR was used to assess the enrichment of ChIP and MeDIP experiments and to validate the integrity of sequencing libraries<br><br><b>FILES</b><br>Validation of ChIP-seq library enrichment:<br><b>20130426-CA1-1h-K27ac-K79me3-K4me3-H3-lib_qPCR.xlsx</b> – this file contains tables with qPCR values from validations of ChIP-seq libraries for samples from CA1 neuronal or non-neuronal cells (+ or -, respectively) taken 1 hour (1h) after naïve (N), context (Ct) or context-shock (FC) and immunoprecipitated for H3K27ac, H3K79me3, H3K4me3 or H3. The target genes were NeuN, Chd4, GAPDH, InterG and cFOS.<br><b>20130715-ACCnaiveK76me3K27ac_qPCR.xlsx</b> – this file contains tables with qPCR values from validations of ChIP-seq libraries for naïve (N) samples from ACC neuronal or non-neuronal cells (+ or -, respectively) and immunoprecipitated for H3K79me3 or H3K27ac. The target genes were NeuN, Chd4 and GAPDH.<br><b>20131125-CA1-Naive_qPCR.xlsx</b> – this file contains tables with qPCR values from validations of ChIP-seq libraries for naïve (N) samples from CA1 neuronal or non-neuronal cells (+ or -, respectively) and immunoprecipitated for H3K27ac, H3K27me3, H3K79me3, H3K4me3, H3 or H3K4me1. The target genes were TFF1, GAPDH, InterG and NeuN.<br><b>20150122-CA1-K9ac_qPCR.xlsx</b> – this file contains tables with qPCR values from validations of ChIP-seq libraries for samples from ACC neuronal or non-neuronal cells (+ or -, respectively) taken 1 hour (1h) after naïve (N), context (Ct) or context-shock (FC) and immunoprecipitated for H3K9ac. The target genes were cFosF2R2, cFosF3R3, GAPDH, InterG and NeuN.<br><br>Validation of MedIP-seq library enrichment:<br><b>20150729-MeDIP-LIB-ACC_qPCR.xlsx</b> – this file contains tables with qPCR values from validations of MedIP-seq libraries for samples from ACC taken 1 hour (1h) after naïve (N), context (Ct) or context-shock (CS). The target genes were Cobl, Loxhd1, Vrk1 and GAPDHenh (-).<br><b>20150802-ACC-lib_qPCR.xlsx</b> – this file contains tables with qPCR values from validations of MedIP-seq libraries for samples from ACC taken 1 hour (1h) or 4 weeks (4w) after naïve (N), context (C) or context-shock (CS). The target genes were Fgfr2, Nova1 and Reelin.<br><br><b>DATA GENERATION</b><br>qPCR experiments were run on Roche LightCycler 480, and the machine was used to export the Ct values. The resulting output was imported into pyQPCR (version 0.10dev) using dilutions of inputs as standards, along with the following parameters:<br>Calculation type: Absolute quantification<br>Confidence interval: 90.00%<br>Machine: Roche LightCycler 480<br>Maximum E(Ct): 0.30<br>Minimum Ct: 35.00<br><br>