Dataset for "Cell Painting PLUS: Expanding the multiplexing capacity of Cell Painting-based phenotypic profiling using iterative staining-elution cycles"

This data set includes all relevant source data (aggregated and processed profiles) as well as image analysis pipelines (Harmony and Cell Profiler software), KNIME pipelines and R code for data analysis and generation of plots for figures that support the key findings of the following study:

Cell Painting PLUS: Expanding the multiplexing capacity of Cell Painting-based phenotypic profiling using iterative staining-elution cycles

 Elena von Coburg1,2, Marlene Wedler1,3, Jose M. Muino1, 4, Christopher Wolff5, Nils Körber6, Sebastian Dunst1, Shu Liu1

1 German Centre for the Protection of Laboratory Animals (Bf3R), German Federal Institute for Risk Assessment (BfR), Berlin, Germany

2 Department of Food Chemistry, University of Potsdam, Potsdam, Germany

3 Institute of Biology, Free University of Berlin, Berlin, Germany

4 Institute of Clinical Pharmacology and Toxicology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

5 Screening Unit, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany

6 Centre for Artificial Intelligence in Public Health Research, Robert Koch Institute, Berlin, Germany

* Equal contribution, correspondence: [e-mail], [e-mail]