scRNAseq analysis comparing primary CD4+ T cells in resting memory state or stimulated with anti-CD3/anti-CD28 states and the effect of loss of ADAP1

By comparing the differenital gene expression of resting memory CD4+ cell and their transition to stimulated states, we are able to interrogate the effects that loss of ADAP1 has on T cell activation. Comparison between DEG of Ctrl cells (resting to stimulated) vs ADAP1 CRISPR cells (resting to stimulated) yielded 515 overlapping DEG (397 upregulated and 118 downregulated). Out of which, 364 of the 397 overlapping upregulated genes induced to significantly lower levels in ADAP1CRISPR cells compared to the level of fold change induction in Ctrl cells. Overall design: Primary CD4+ T cells of WT (Ctrl) or cells subjected to ADAP1 CRISPR/Cas9 (KD) were left resting or stimulated with anti-CD3/anti-CD28 for 4 hrs