Microscopy Data for "A single-vesicle fluorescence microscopy platform to quantify phospholipid scrambling"

TIRF microscopy image data. for all files: uneven number is lipid channel tiffstack, next even number is corresponding protein channel tiffstack. SDT_Data: tiff files for Dithionite assay (Extended Data Figure 5) each stack consists of 9 images: image 1-3 : 'before' images image 4-6 : after Dithionite addition image 7-9 : after Alamethicin addition Scramblase_assay_Liposome_controls: tiffstacks for scramblase assay controls with liposomes, Protein channel reduced to 10 images (no bleaching). Figure 2A-C; Liposome controls: head-labeled, asymmetric-labeled and symmetric-labeled Extended Data Figure 3A-C; Liposome controls: flow speeds (low, middle, high) temperature (24, 33 and 40 degree) reporter lipdis (14PC, 16PC, 14PE, 16PE, 16PS, SM)) Scramblase_assay_L+NCL+pCL: tiffstacks for scramblase assay (Liposomes (L), non crosslinked (NCL) and purified crosslinked (pCL); Figure 3A-F, Extended Data Figure 6E-G) Protein channel reduced to 10 images for easier, rough analysis of protein occupancy based on initial protein signal and not PBSA. Exemplary files with complete PBSA files for pCL sample (Figure 3G-j, Extended Data Figure 7) are uploaded in Exemplary_Scramblase_assay_pCL_with_PBSA.zip and rest is uploaded in Scramblase_assay_pCL_with_PBSA.zip and cramblase_assay_pCL_with_PBSA_2.zip. Scramblase_assay_Opsin: tiffstacks for scramblase assay with complete PBSA (Opsin sample; Extended Data Figure 9E-G). Code: code for microscopy image analysis and bulk figures. exemplary SDT and kinetic assay data with corresponding output plots.