Validation of human sensory neuron differentiation method_Fig1D

Human whole transcriptome AmpliSeq analysis was completed on four samples to compare gene expression levels of markers of different cell types. Samples were collected from induced pluripotent stem cell (iPSC) starting material as well as Day 18 and Day 28 of a new differentiation method that combines small molecules with the transcription factor Neurogenin 3 (NGN3) to induce sensory neuron specification. A fourth sample was collected from a small molecule-only differentiation at 8 weeks. Normalized reads per million from the iPSC, Day 18, and Day 28 samples were used to show loss of markers of pluripotent cells and specification of sensory neurons over time in Figure 1D of the manuscript. The three datafiles are: 1) Human whole transcriptome AmpliSeq normalized reads per million gene count file; 2) Gene list from Figure 1D; 3) Meta data file listing barcode IDs and reference genome information.