Multimodal single nuclei profiling of human macular and peripheral retinal pigment epithelial cells

We sought to understand how the RPE confers region-specific disease susceptibility in the retina by examining heterogeneity within human donor RPE. We profiled RPE nuclei from the macular and peripheral retina using joint single-cell RNA and ATAC sequencing. Overall design: RPE nuclei were isolated from macular and peripheral regions of the posterior pole from four healthy human donors. Joint profiling of nuclear chromatin and transcript abundance was performed.