Peptides used in the peptide array binding assay with GF14 isoforms.

For all 14-3-3 target proteins, the interaction with Arabidopsis GF14 proteins was demonstrated. *, peptides reproducing 14-3-3 binding site identified in Arabidopsis 14-3-3 clients. **, peptides gathered from an alignment of identified binding sequence of 14-3-3 clients derived from different plant species and the corresponding Arabidopsis targets. All the other peptides reproduced putative 14-3-3 binding site, present in the indicated Arabidopsis 14-3-3 target, and chosen based on their similarity with the 14-3-3 mode I or mode II binding motif.NR, nitrate reductase; TPS5, trehalose phosphate synthase 5; F2KP, fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase; SPS, sucrose-phosphate synthase; GS1, glutamine synthetase 1; GS2, glutamine synthetase 2; GADPH, glyceraldehydes-3-phosphate dehydrogenase; BZR1, brassinazole-resistant 1 protein; TPK1, two pore K+ channel 1; CPK1, calcium-dependent protein kinase 1; phot1, phototropin 1; phot2, phototropin 2; ABF3, ABA-responsive-element Binding Factor 3; SERK1, somatic embryogenesis receptor-like kinase 1; KAPP, kinase associated protein phosphatase.So, Spinacia oleracea; Bo, Brassica oleracea; Vf, Vicia faba.