Tigecycline among clinically significant multidrug resistant pathogens

Purpose: Tigecycline, a glycylcycline antibiotic is a promising option for the treatment of single or multidrug-resistant pathogens. The aim of the study was to evaluate the in-vitro Tigecycline susceptibility of various pathogens from clinical samples received at tertiary care hospitals in South India. 
Methods: The analysis of specimens from patients admitted was carried out in this retrospective cross-sectional study. The identification and antimicrobial susceptibility testing were performed by semi-automated Vitek 2 systems and Kirby Bauer method. The pattern of data analysis was done by descriptive statistics.
Results: Among 2,574 isolates, 812 isolates were gram-positive pathogens and 1762 isolates were gram-negative pathogens. Resistance to Tigecycline was more common among gram-negative pathogens (18.62%) in comparison to the gram-positive pathogens (0.49%). Among 740 Extended Spectrum Beta Lactamases (ESBL) producers such as Klebsiella species & E. coli, 629 isolates w..., The comprehensive data which includes 2,574 clinically significant isolates were retrieved from Laboratory Information System and microbiology records from the KMC hospital.
Study participants: The data of all samples received at Kasturba Medical College microbiology department were collected for a period of one year except for urine specimens as urinary concentration of tigecycline is poor.
The positive cultures were identified and antibiotic susceptibility testing was done for tigecycline by Vitek 2 compact system and Kirby-Bauer Disk Diffusion method as per the manufacturer's & FDA-approved guidelines respectively and interpreted as sensitive & resistant. EUCAST criteria were used for interpretation of MIC which ranged from 0.06 to 64 and Disk diffusion with 15 μg (Hi-Media). As per these criteria, MIC of tigecycline for Enterobacteriaciae < 2ug/ml is sensitive, and > 8ug/ml was considered resistant. By the Disk Diffusion method, the isolates with >19mm & < 14..., Microsoft Excel,