Super-fast generation of all-female grass carp via transplantation of female germline stem cell into zebrafish

We characterized and purified female germ line stem cells (GSCs) from the ovaries of grass carp fry three months after fertilization. Transplanting female grass carp GSCs into zebrafish larvae with depleted germ cells to generate replacement zebrafish. Transplanted female grass carp GSC underwent accelerated spermatogenesis in zebrafish receptors. Three months after transplantation, the zebrafish recipient developed into a male capable of producing fully X-shaped sperm from the donor grass carp female GSC. Transplanting female grass carp GSCs into zebrafish larvae with depleted germ cells to generate replacement zebrafish. Transplanted female grass carp GSC underwent accelerated spermatogenesis in zebrafish receptors. Three months after transplantation, the zebrafish recipient developed into a male capable of producing fully X-shaped sperm from the donor grass carp female GSC.

本研究对受精后三个月草鱼鱼苗的卵巢中的雌性生殖系干细胞（female germ line stem cells，GSCs）进行了鉴定与纯化。将雌性草鱼GSCs移植至生殖细胞缺失的斑马鱼幼体中，以构建替代型斑马鱼。移植的雌性草鱼GSCs在斑马鱼受体内发生了加速的精子发生过程。移植后三个月，斑马鱼受体发育为雄性个体，可产生源自供体雌性草鱼GSC的完整X形精子。将雌性草鱼GSCs移植至生殖细胞缺失的斑马鱼幼体中，以构建替代型斑马鱼。移植的雌性草鱼GSCs在斑马鱼受体内发生了加速的精子发生过程。移植后三个月，斑马鱼受体发育为雄性个体，可产生源自供体雌性草鱼GSC的完整X形精子。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于通过移植雌性草鱼生殖干细胞到斑马鱼体内，实现快速生成全雌性草鱼的技术。研究展示了移植后斑马鱼受体能够产生来自草鱼的X型精子，为性别控制和快速繁殖提供了新方法。数据集包含206.91 GB的实验数据，共18个文件。