FabT_S.pyogenes_Metabolomic analysis

In Streptococcus pyogenes, the type II fatty acid (FA) synthesis pathway FASII is feedback-controlled by the FabT repressor. A FabT mutation leads to bacterial growth defect in the presence of eukaryotic cells and their supernatant. We investigated a possible metabolic basis for the mFabT growth defect, which could reflect an incapacity to use cell-secreted products for growth. We used a metabolomics approach to assess metabolites that are differentially consumed by mFabT compared to the WT strain, cultivated in the presence of non-infected endometrial cells supernatants (HEC-1-A cells). HEC-1-A supernatants were inoculated or not with WT or mFabT strains during 8 or 16 h. The metabolite composition of these supernatants was analyzed by Proteigene (https://proteigene.com) using MxP® Quant 500 kit (Biocrates) by two analytical methods, LC-MS/MS for small molecules and FIA-MS/MS for lipids. This analysis was repeated on 3 independent series of supernatants. These analyses have a defined detection threshold (LOD) for each family of metabolite. You can found in attachment the raw folders and sample informations.