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Single-cell targeted transcriptomic analysis (CITEseq) in CD22 CART infusion products

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NIAID Data Ecosystem2026-05-02 收录
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Cryopreserved CAR T-cells were thawed at 37 degree and resuspended in the sample buffer. SIngle-cell capture, and cDNA library preparation were performed using the BD Rhapsody Express SIngle-cell analysis system according to the manufacturer's instructions. An Abseq panel containing 30 surface markers and a customized immune response panel (398 genes) were employed. Paired-end sequencing was conducted on Illumina Nextseq550 platform.

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2024-11-15
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