Gene regulation of candidate cancer gene DIP2C

Purpose: The disco-interacting protein 2 homolog C (DIP2C) gene is an uncharacterized gene found mutated in breast and lung cancers. We want to understand the role of DIP2C in tumor development. Methods: We engineered human DIP2C knockout cell systems by genome editing, and then use next-generation sequencing to identify the genes affected by the loss of DIP2C. Results: Inactivation of DIP2C triggers substantial gene expression changes, cellular senescence and epithelial to mesenchymal transition in cancer cells. Overall design: To investigate if loss of DIP2C affects gene expression we performed RNA sequencing and compared gene expression levels in the DIP2C knockout cells to those in parental RKO cells.