Relative specific infectivities of virus particles from nsp1 mutants with imbalanced mRNA profiles.

aVirus titers were determined by plaque assays of culture supernatants harvested at 24 h post-transfection and normalized to the pfu/ml value of the wt control, which was set at 1.bEAV genomic RNA levels were quantified in virion preparations obtained from culture supernatants harvested at 24 h post-transfection by reverse transcription and quantitative PCR. Values for mutant virions were obtained by comparing their threshold cycle (Ct) against the qPCR standard curve, and were normalized relative to the genomic RNA level in the wt control, which was set at 1.cRelative specific infectivity values were calculated by dividing the relative infectivity (mutant∶wt pfu ratio) by the relative genomic RNA content for each construct.dThe data shown are derived from two independent experiments.