RNA sequencing analysis of in vitro-derived early spinal cord progenitors

human embryonic stem cells (hESCs) were treated with WNT and FGF agonists to generate NMP-like cells (D3) which were then subsequently treated with WNT and high levels of FGF signalling to produce early spinal cord progenitors (D7 of differentiation) Gene expression profiling utilised total RNA extracted from hECS cells (N=3); D3 axial progenitors (N=3); D7 spinal cord progenitors (N=3)