Single-cell transcriptomic analysis of sorted CD8+ T cell subsets isolated from the spleen of C57BL/6 mice treated with aCD137 or an Ig control.

We report the single cell RNA sequencing of mouse CD8+ T cells sorted from the spleen into three differentiation subsets: effector, precursor exhausted and terminally exhausted. CD8 T cells from mice treated with anti-CD137 or an isotype control are compared. Five C57BL/6 mice were treated with anti-CD137 (10ug, clone 3H3) twice a week for 14 days. Ten C57BL/6 mice were treated with an Ig control (100ug, clone HRPN) also twice a week for 14 days. Mice were sacrificed and spleens were harvested for T cell sort. Teff cells were sorted as CD44hiPD1-, precursor exhausted T cells were sorted as CD44hiPD1+Slamf6+Tim3-, and exhausted T cells as CD44hiPD1+Slamf6-Tim3+.