Molecular Insights into PCB Neurotoxicity: Comparing Transcriptomic Responses across Dopaminergic Neurons, Population Blood Cells, and Parkinson's Disease Pathology

In this study, we conducted a comprehensive assessment of the transcriptomic responses in iPSC-derived dopaminergic neurons upon exposure to different concentrations (0.01 µM, 0.5 µM, and 10 µM) of PCBs. Specifically, we focused on PCB-180, a congener known for its relatively high accumulation in human brains. The exposure durations were 24 hours and 74 hours, allowing us to capture both short-term and more prolonged effects on gene expression patterns. Overall design: First, we differentiated human iPSCs into dopaminergic neurons. Cells were examined using temporal transcriptomics analysis at various time-points after 24h of exposure to the potential neurotoxin PCB-180.