Unr is a potential post-transcriptional master regulator of protein expression

Ribonucleoprotein immunoprecipitation followed by mass spectrometry and RNA-Seq was used to investigate the 'Upstream of N-RAS' (UNR) protein interactome across three cancer cell lines. A number of novel UNR-interacting proteins and transcripts were discovered, including the ubiquitin E3 ligase, HUWE1; the nucleolar protein, NARR; the multifunctional protein, SQSTM1; and the erythropoiesis-related protein, LDB1 . GO-term analysis of putative UNR interactors suggested that UNR interacts with both proteins and mRNAs encoding proteins that are involved in RNA binding Overall design: Examination of UNR binding to RNA by RIP-RNA-Seq using three replicates of three cell types,