High frequency of Enterovirus E, Bovine Kobuvirus and Astrovirus in fecal samples from the cattle in Central Colombia

The application of metagenomic sequencing techniques provided valuable insights into the virome present in bovine fecal samples and emphasized the importance of virological surveillance in veterinary research. Our study revealed a consistent and uniform composition of viral communities in Holstein breed or crossbreeds of Normado-Holstein across different municipalities at the same region, suggesting similar bio-sanitary, care, and climatic conditions, as well as a high commercial flow in the region. The predominance of commonly. The predominance of commonly found fecal viruses and the low abundance of confirmed pathogens may contribute to this homogeneity. We identified several viruses of veterinary importance, including BoAstV, EVE, and BKV, which were reported for the first time in Colombia. Additionally, the presence of a CRESS Virus in animal feces was confirmed. Our findings shed light on the frequency of these potentially pathogenic viruses and emphasize the need for further research to establish possible correlations with disease occurrence by considering associated metadata, such as symptom presentation. Based on the abundance and frequency of these viruses, we recommend that diagnostic testing, whether it is routine or non-routine, should include these viruses as targets. By doing so, it ensures the early detection and timely intervention of markers of interest in the population. Additionally, incorporating these targets will prove valuable in specific situations such as suspected pathogen cases, outbreak investigations, or research studies where a comprehensive approach to disease detection is required. This will contribute to a better understanding of their impact on animal health and the economic activity associated with livestock production.