The prevalence of human cytomegalovirus in colorectal cancer and viral gene expression profiles. The prevalence of human cytomegalovirus in colorectal cancer and viral gene expression profiles

Five HCMV (+) CRC tissues detected by PCR were selected for RNA-seq. GEPs were pre-processed by Cutadapter and FastQC to remove jointed reads and low-quality reads. Tophat (v.2.0.0) was used to compare the sequences with Homo sapiens and HCMV genomes. The GEPs of HCMV was determined by Integrated Genomics Viewer (IGV) and Partek® Genomics Suite™ (version 6.5 beta, Partek Inc., St. Louis, MO, USA). Overall design: HCMV gene expression profiles in 5 CRC tissues by transcriptome-sequencing.