Nitrates increase abscisic acid levels to regulate haustoria formation in the parasitic plant Phtheirospermum japonicum. Nitrates increase abscisic acid levels to regulate haustoria formation in the parasitic plant Phtheirospermum japonicum

Transcriptomic changes during haustoria formation in the parasitic plant Phtheirospermum japonicum. We performed RNAseq on Phtheirospermum japonicum seedling infecting and not infecting over 5 time points during haustoria formation for the control treatment and over 3 time points for the ammonium nitrate and 6-Benzylaminipurine. We investigated the transcriptomic changes during haustoria formation and how this is affected in the transcritpome level by nitrate and cytokinin treatment. Overall design: transcriptomic changes in the root of 7 days old Phtheirospermum japonicum (hasutorium formation site +/- 2mm) that has been infecting 7 days old Arabidopsis thaliana (Col-0). Samples taken at 0,12,24,48,72 hours post infection (hpi). Infecting plants were compared to not-infecting plants. For ammonium nitrate and 6-Benzylaminopurine samples were collected for 0,12,24 hpi.