Heterogeneity of circulating CD4+CD8+ double-positive T cells characterized by scRNA-seq analysis and trajectory inference

We subdivided circulating CD4+CD8+ double positive T cells, known to be associated with various disease environments, into several subtypes through single-cell RNA sequencing, and analyzed transition in transcriptome using trajectory analysis. Overall design: Heterogeneous subsets of circulating CD4+CD8+ double positive T cells were analyzed by scRNA-seq after labeling CD4 and CD8 feature barcodes, characterized their characteristic with CD4, CD8 coreceptor expresion levels.