Quality control of psychoactive substances inhuman breastmilk samples from a humanmilk bank by disposable pipetteextraction–gas chromatography–massspectrometry: supplementary material

The lack of data regarding infant exposure to drugs of abuse consumed by lactating mothers has become
a major health concern. Investigating psychoactive substances and their metabolites in breastmilk is an
analytical approach to estimate the rate at which drugs of abuse are excreted and how much infants
are exposed to them. In this study, we have developed and validated a GC–MS method using disposable
pipette tips for simultaneously extracting ten analytes from breastmilk samples obtained from a milk bank
in the city of Ribeir ˜ao Preto, Brazil. All the 67 analyzed samples tested negative for all the analytes. This
is the first study that has applied disposable pipette extraction to analyze drugs of abuse in breastmilk
samples.