Characterization of iPSC-derived macrophages (surface marker profile, Figure 5)

Characterization of surface markers expressed on iPSC-derived macrophages produced in stirred tank bioreactors (method described in Ackermann et al., Nature Communcations, 2018) Conclusion: Macrophages produced from hCD34iPSC in stirred tank bioreactors show a highly pure surface maker profile of CD45+/CD11b+/CD14+/CD163+/TRA-1-60-. Calibration of baseline settings, PMT-Voltages and quality control of the LSR II cytometer was regularly performed using the Becton-Dickinson CS&T beads and FACSDiva software according to the manufacturer.