Transcriptomic profile of brain endothelial cells after stroke w/o chronic stress

Our previous studies demonstrate that chronic stress leads to worse stroke outcome with increased brain lesion sizes after experimental brain ischemia. These effects were mediated by endothelial dysfunction and altered cerebral blood flow regulation. RNA-sequencing was performed to further investigate the underlying molecular mechanisms of stress-induced endothelial dysfunction. Overall design: Chronic stress and control animals were exposed to 30 min MCAo followed by reperfusion. At 72h after stroke endothelial cells from the MCAo territory and the corresponding contralateral tissue were isolated. Brain tissue from 3-5 animals were pooled per condition and 4 replicates per condition were used for RNA-Sequencing.