Data from: Testosterone regulates CYP2J19-linked carotenoid signal expression in male red-backed fairywrens (Malurus melanocephalus)

The first data set “Khalil_etal_2020_HPLC_data.csv” is the circulating carotenoid data from the High Performance Liquid Chromatography (HPLC) Analysis. This data was used for the circulating kecarotenoid model. The data set consists of (in this order): Year of sample collection Sample number Day of sample collection ABBBS number – metal band number, a unique identifier Color bands – color band combination, another unique identifier, and the one used in the model for the random effect Sex Age - those with a “+” were of minimum age, since they are considered at least that age. Those without a “+” are of known age. Age_min – same information as age, but without the “+” to be used in analysis. When running the model with only known age birds (Table S1), we filtered out individuals that had a “+” in the Age column. The next six columns are the circulating carotenoids -ador (alpha-doradexanthin), asta (astaxanthin), lut (lutein), adoni (adonirubin), zea (zeaxanthin), and canth (canthaxanthin). Breeding_phenotype – the breeding phenotype (as of November 1st) used for the analysis. F is female, DM is unornamented male, and BM is ornamented male. The second data set “Khalil_etal_2020_qpcr_data.xlsx” is the qPCR ct data from the CYP2J19 and GAPDH expression analysis. This data was used to calculate log fold change using the delta-delta ct method described in text. The data set consists of three sheets: 1) gapdh_ct – this is the ct data using the GAPDH primers. This includes The well the sample was in The cq The sample number The phenotype of the sample. F is female, T-DM is testosterone-implanted unornamented male, DM is control unornamented male, and BM is ornamented male. The average GAPDH ct, calculated from the cq column as each sample was run in triplicate 2) cyp2j19_ct – this is the ct data using the CYP2J19 primers. This includes the same type of information as the gapdh_ct sheet 3) delta_delta_ct_math – this is all the math to calculate the log fold change using the delta delta ct method. This includes: The sample number The phenotype If the control unornamented male (DM) had a sham implant or no implant The average GAPDH ct from sheet 1 The average CYP2J19 cqtfrom sheet 2 The delta ct (CYP2J19 ct – GAPDH ct) The average delta ct for females. This is used as our “calibrator” sample, as described in the supplementary methods Delta delta ct (delta ct – average female delta ct) Fold change (2^delta delta ct) Log fold change. This value is used in the ANOVA and in figures. 4) Intra-assay CV - this is the math for how intra-assay coefficient of variation for the GAPDH and CYP2J19 qPCR assays