PS I and PS II activities of WT and the RpaA mutant.

Thylakoid membranes were isolated from cells grown in LL and the transferred to HL for 24 h. The activities of PSI and PSII were evaluated by measuring oxygen evolution resulting from electron transport from DCIP/ascorbic acid via PS I to MV and from H2O via PSII to phenyl-p-benzoquinone, respectively. Thylakoid membranes were added to the reaction mixture to the final concentration of 15 g/ml of chlorophyll. Data were generated by averaging three independent experiments on different days.DCPIP- 2,6-Dichlorophenolindophenol; MV- Methyl viologen.