DNA hydroxymethylation profiles reveal possible role of highly methylated TLR signaling on Fasciola gigantica excretory/secretory products (FgESPs) modulation of buffalo DCs [hMeDIP-seq]

Purpose: To investigate whether FgESP affects the hydroxylmethylation level of DCs Methods: we have profiled the DNA hydroxymethylome by using hydroxymethylated DNA immunoprecipitation sequencing (h)MeDIP-Seq in the current study Results: DNA hydroxymethylation profiles revealed significant changes in the 5-hydroxymethylcytosine (5hmC) levels in 360 genes when DCs were treated with FgESPs. These differentially expressed genes were highly enriched in pathways associated with immune function. Conclusion: For the first time, our study provides a genome-wide mapping of DNA hydroxylmethylation for DCs that interacted with FgESPs. In the current study, we have profiled the DNA hydroxymethylome by using high-throughput sequencing. Total DNA was extracted from buffalo PBMC DCs that were treated with FgESP, which was followed byFgESPs, and then performed hydroxymethylated DNA immunoprecipitation sequencing (h)MeDIP-Seq.