Genome-wide CRISPR/Cas9 screen identifies regulators of BCMA expression on multiple myeloma cells

To search for BCMA regulators, we carried out a genome-wide CRISPR/Cas9 screen in the OPM2 and MOLP8 MM cell lines, which express BCMA. We transduced cells with the Brunello library, containing 76,441 small guide RNA (sgRNA) sequences targeting 19,114 genes. We isolated BCMA-high-expressing (BCMAhi) and BCMA-low-expressing (BCMAlo) cells by fluorescence-activated cell sorting and assessed sgRNA representation using massively parallel sequencing.