In vivo differentially-expressed genes in Peromyscus leucopus, Mus musculus, and Rattus norvegicus blood in response to LPS

Animals that are competent reservoirs of zoonotic pathogens commonly suffer little morbidity or apparent pathology from the infections. To investigate mechanisms of this tolerance of infection, we used single-dose lipopolysaccharide (LPS) as an experimental model of inflammation and compared the four hour post-injection responses by three rodents: the cricetine rodent Peromyscus leucopus (LL stock), the white-footed deermouse and reservoir for agents of Lyme disease and other zoonoses, and the murids Mus musculus (outbred CD-1 breed), the house mouse, and Rattus norvegicus, the brown rat (Fischer strain). The data presented here are part of a follow-up study of this phenomenon that was previoiusly reported with the title \"An Infection-Tolerant Mammalian Reservoir for Several Zoonotic Agents Broadly Counters the Inflammatory Effects of Endotoxin\" (https://doi.org/10.1128/mBio.00588-21). The project and the description of the samples are described under the following NCBI BioProjects:PRJN..., Adult animals of the species Peromyscus leuopus LL stock, Mus musculus (CD-1 breed) or Rattus norvegicus Fischer strain were inoculated into the peritoneum (IP) with solution of purified E. coli lipopolysaccharide (10 microgram/gram body mass for P. leuocpus and M. musculus and 5 microgram or 20 microgram for R. norvegicus. Controls received saline alone. P. leucopus and M. musculus were in the same experiment and there were 10 animals (5 of each sex) in the treatment group and 10 animals (5 of each sex) in the control group. The rats were studied in a separate experiment with 5 control animals and 6 animals receiving 5 microgram LPS per gram and 5 animals receiving 20 micrograms LPS per gram. In both experiments 4 hours after LPS or control injections the animals were humanely euthanized and blood collected for complete blood count and for RNA extraction. The extracte RNA was subjected to stranded cDNA library preparation and then Illumina technology (Novaseq) as described in https://d..., The data is in CSV tabular data format and can be opened as a text file or as a spreadsheet.