UVDE-seq mapping of UV-induced 6-4PPs and atypical TA photoproducts

We used a novel high-throughput sequencing method known as UVDE-seq to map the formation of UV-induced 6-4 photoproducts (6-4PP) and atypical thymidine-adenine (TA) photoproducts at single nucleotide resolution across the yeast genome in wild-type and rad16 mutant cells, which are deficient in GG-NER. We also analyzed 6-4PP and TA photoproducts in UV-irradiated yeast genomic DNA (i.e., naked DNA). Overall design: Mapping UV-induced 6-4PPs and TA photoproducts in wild-type BY4741 yeast cells, mutant yeast cells deleted for the RAD16 gene. (i.e., rad16?), and isolated yeast genomic DNA. Yeast cells were irradiated with 600J/m2 of UVC, while the isolated genomic DNA was irradiated with either 400 or 500 J/m2 of UVC.