mRNA expression profile of mouse CD4 T cells (treated vs. untreated with PM2.5 suspension)
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP175258
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资源简介：
Purpose: PM2.5 can impact mouse TH17 cell differentitation. To Investigator the genes altered by PM2.5 during TH17 differentiation, mRNA-seq was performed. Methods: Naïve T cells were isolated from mouse spleen by magnetic beads. These naïve T cells were stimulated by CD3/CD28 antibodies and cultured under TH17 polarization conditions for 4 days before harvested. For PM2.5 group, 100ug/ml of PM2.5 suspensions were added to culture medium 6 hours after stimulation. The total RNA was extracted using the TRIzol reagent (Invitrogen). The library construction and sequencing was performed by Illumina Xten at Shanghai Biotechnology Corporation. Overall design: PM2.5 treated group was compared to control group. Each group has 3 samples.
创建时间：
2019-07-01
